Interaction Details

Details of experiments between Q8TCU6 and P0DTC9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PREX1
Fragment: 608-718
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov2-N
Fragment: 410-419
Site: PBM
Sequence: SMSSADSTQA
Construct: Biotin-(PEG)3-QQSMSSADSTQA

Measured dissociation constant (10^-6M):

Standard deviation of dissociation constant (10^-6M): 0

Average holdup BI: -0.01

Holdup BI standard deviation: 0.02

Normalized holdup BI: 0

Normalized holdup BI standard deviation: 0

Immobilized partner concentration (10^-6M): 20

P value: 0

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experiment series 2