Interaction Details


Details of experiments between Q8TBB1 and Q9P2E8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LNX1
Fragment: 371-470
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MARCHF4
Fragment: 401-410
Site: PBM
Sequence: RELVMRVTTV
Construct: biotin-ado-ado-RELVMRVTTV
Average holdup BI: 0.39
Normalized holdup BI: 0.39
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.58
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: LNX1
Fragment: 261-371
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MARCHF4
Fragment: 401-410
Site: PBM
Sequence: RELVMRVTTV
Construct: biotin-ado-ado-RELVMRVTTV
Average holdup BI: 0.09
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: LNX1
Fragment: 500-596
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MARCHF4
Fragment: 401-410
Site: PBM
Sequence: RELVMRVTTV
Construct: biotin-ado-ado-RELVMRVTTV
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.