Interaction Details


Details of experiments between Q8TBB1 and Q9HD26
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LNX1
Fragment: 719-728
Site: PBM
Sequence: TIVSWPGTFL
Construct: Biotin-Ahx-TIVSWPGTFL

Protein B

Protein: GOPC
Fragment: 278-373
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 36115835