Interaction Details


Details of experiments between Q8TBB1 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LNX1
Fragment: 719-728
Site: PBM
Sequence: TIVSWPGTFL
Construct: Biotin-Ahx-TIVSWPGTFL

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.7
Normalized holdup BI: 0.77
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.34
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: LNX1
Fragment: 261-371
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NHERF3
Fragment: 510-519
Site: PBM
Sequence: SSSNSEDTEM
Construct: Biotin-Ahx-SSSNSEDTEM
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: LNX1
Fragment: 371-470
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NHERF3
Fragment: 510-519
Site: PBM
Sequence: SSSNSEDTEM
Construct: Biotin-Ahx-SSSNSEDTEM
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: LNX1
Fragment: 500-596
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NHERF3
Fragment: 510-519
Site: PBM
Sequence: SSSNSEDTEM
Construct: Biotin-Ahx-SSSNSEDTEM
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: LNX1
Fragment: 719-728
Site: PBM
Sequence: TIVSWPGTFL
Construct: Biotin-Ahx-TIVSWPGTFL

Protein B

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: LNX1
Fragment: 719-728
Site: PBM
Sequence: TIVSWPGTFL
Construct: Biotin-Ahx-TIVSWPGTFL

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835