Interaction Details


Details of experiments between Q8TAA9 and Q96RT1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: LAP2
Fragment: 1314-1412
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.07
PUBMED ID: 36115835