Interaction Details


Details of experiments between Q8TAA9 and Q86UL8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.3
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.47
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Normalized holdup BI: 0.3
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.42
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.05
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835