Interaction Details


Details of experiments between Q8TAA9 and Q15599
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: NHERF2
Fragment: 142-238
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.09
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 36115835