Interaction Details

Details of experiments between Q8TAA9 and P53671
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: VANGL1
Fragment: 515-524
Site: PBM
Sequence: VLRLQSETSV
Construct: biotin-ttds-VLRLQSETSV

Protein B

Protein: LIMK2
Fragment: 143-248
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.24

Normalized holdup BI: -0.24

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835