Interaction Details


Details of experiments between Q8NI35 and Q9H1D0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: Inadl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: 0.76
Normalized holdup BI: 0.76
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.33
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: Inadl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: 0.3
Normalized holdup BI: 0.3
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.41
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: Inadl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: 0.14
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.97
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: Inadl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: 0.11
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 5

Protein A

Protein: Inadl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 6

Protein A

Protein: Inadl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: 0.02
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 7

Protein A

Protein: Inadl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 8

Protein A

Protein: Inadl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: -0.04
Normalized holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 9

Protein A

Protein: Inadl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: TRPV6
Fragment: 756-765
Site: PBM
Sequence: EDGESWEYQI
Construct: biotin-ado-ado-EDGESWEYQI
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.