Interaction Details


Details of experiments between Q8NI35 and K9N5R3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: Inadl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.25
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.23
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: InaDl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0.15
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.02
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: InaDl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.9
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: Inadl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.09
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.1
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.81
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: InaDl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0.09
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.75
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: Inadl
Fragment: 1058-1167
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.1
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 3.75
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: Inadl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.09
Normalized holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.74
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: InaDl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.64
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 9

Protein A

Protein: Inadl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.07
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.61
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: Inadl
Fragment: 1530-1615
Site: PDZ_9
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.57
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: Inadl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.55
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: Inadl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.04
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.55
PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: InaDl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.51
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 14

Protein A

Protein: InaDl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 15

Protein A

Protein: InaDl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 16

Protein A

Protein: InaDl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 17

Protein A

Protein: InaDl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 73-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: Biotin-ado-ado-KPPLPPDEWV
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 18

Protein A

Protein: Inadl
Fragment: 113-229
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: -0.11
Holdup BI standard deviation: 0.17
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 19

Protein A

Protein: Inadl
Fragment: 1219-1332
Site: PDZ_7
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: -0.05
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 20

Protein A

Protein: Inadl
Fragment: 1425-1530
Site: PDZ_8
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.03
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 21

Protein A

Protein: Inadl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 22

Protein A

Protein: Inadl
Fragment: 1673-1763
Site: PDZ_10
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: -0.05
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 23

Protein A

Protein: Inadl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 24

Protein A

Protein: Inadl
Fragment: 238-335
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: -0.07
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 25

Protein A

Protein: Inadl
Fragment: 359-469
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.02
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 26

Protein A

Protein: Inadl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 27

Protein A

Protein: Inadl
Fragment: 547-651
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: -0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 28

Protein A

Protein: Inadl
Fragment: 673-780
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MERS-E
Fragment: 71-82
Site: PBM
Sequence: KPPLPPDEWV
Construct: biotin-DSKPPLPPDEWV
Average holdup BI: 0.03
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835