Interaction Details

Details of experiments between Q8N448 and Q9Y6V0
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: LNX2
Fragment: 681-690
Site: PBM
Sequence: TVICWPGSLV
Construct: Biotin-Ahx-TVICWPGSLV

Protein B

Protein: PCLO
Fragment: 4484-4598
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Average holdup BI: 0.01

Normalized holdup BI: 0.01

Immobilized partner concentration (10^-6M): 18

Experiment method: DAPF_holdup

Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout

Number of measurements: 1

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835

Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.