Interaction Details


Details of experiments between Q8N448 and Q8TDW7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV
Average holdup BI: 0.08
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.75
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV
Average holdup BI: -0
Holdup BI standard deviation: 0.02
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835