Interaction Details


Details of experiments between Q8N448 and Q13424
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LNX2
Fragment: 681-690
Site: PBM
Sequence: TVICWPGSLV
Construct: Biotin-Ahx-TVICWPGSLV

Protein B

Protein: SNTA1
Fragment: 84-171
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.96
PUBMED ID: 36115835
Experimental Details

The peptide stock solution had solubility issues. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.