Interaction Details


Details of experiments between Q8N448 and P0DTC4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: 0.29
Normalized holdup BI: 0.32
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: 0.25
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
Measured pKd: 4.23
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: LNX2
Fragment: 223-326
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: -0.12
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: -0.04
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: LNX2
Fragment: 592-688
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-CoV2-E
Fragment: 66-75
Site: PBM
Sequence: NSSRVPDLLV
Construct: biotin-NLNSSRVPDLLV
Average holdup BI: 0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835