Interaction Details


Details of experiments between Q8N448 and A4U7F2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.07
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.6
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: LNX2
Fragment: 592-688
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835