Interaction Details


Details of experiments between Q8N1I0 and Q9NZN5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DOCK4
Fragment: 1957-1966
Site: PBM
Sequence: RPLPRKVSQL
Construct: biotin-ttds-RPLPRKVSQL

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.36
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.82
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DOCK4
Fragment: 1957-1966
Site: PBM
Sequence: RPLPRKVSQL
Construct: biotin-ttds-RPLPRKVSQL

Protein B

Protein: ARHGEF12
Fragment: 67-151
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.37
Normalized holdup BI: 0.41
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.62
PUBMED ID: 36115835