Interaction Details


Details of experiments between Q8N1I0 and A4D2P6
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DOCK4
Fragment: 1957-1966
Site: PBM
Sequence: RPLPRKVSQL
Construct: biotin-ttds-RPLPRKVSQL

Protein B

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.31
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: DOCK4
Fragment: 1957-1966
Site: PBM
Sequence: RPLPRKVSQL
Construct: biotin-ttds-RPLPRKVSQL

Protein B

Protein: GRID2IP
Fragment: 272-370
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Normalized holdup BI: 0.21
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.19
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: DOCK4
Fragment: 1957-1966
Site: PBM
Sequence: RPLPRKVSQL
Construct: biotin-ttds-RPLPRKVSQL

Protein B

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: DOCK4
Fragment: 1957-1966
Site: PBM
Sequence: RPLPRKVSQL
Construct: biotin-ttds-RPLPRKVSQL

Protein B

Protein: GRID2IP
Fragment: 1-87
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835