Interaction Details


Details of experiments between Q8N103 and Q9P202
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TAGAP
Fragment: 722-731
Site: PBM
Sequence: QFQYAKESYI
Construct: biotin-ttds-QFQYAKESYI

Protein B

Protein: DFNB31
Fragment: 263-379
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.47
Normalized holdup BI: 0.47
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.74
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: TAGAP
Fragment: 722-731
Site: PBM
Sequence: QFQYAKESYI
Construct: biotin-ttds-QFQYAKESYI

Protein B

Protein: DFNB31
Fragment: 136-226
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.23
Normalized holdup BI: 0.23
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: TAGAP
Fragment: 722-731
Site: PBM
Sequence: QFQYAKESYI
Construct: biotin-ttds-QFQYAKESYI

Protein B

Protein: DFNB31
Fragment: 813-904
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835