Interaction Details


Details of experiments between Q8IYB3 and P49418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: SRRM1
Fragment: 567-581
Site: PxxP_6
Sequence: PRRRRTPTPPPRRRT
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.86
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.63
PUBMED ID: 38995680

Experiment series 2

Protein A

Protein: SRRM1
Fragment: 567-581
Site: PxxP_6
Sequence: PRRRRTPTPPPRRRT
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.86
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.63
PUBMED ID: N

Experiment series 3

Protein A

Protein: SRRM1
Fragment: 557-571
Site: PxxP_5
Sequence: RRRSPSPAPPPRRRR
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.84
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 5.55
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: SRRM1
Fragment: 557-571
Site: PxxP_5
Sequence: RRRSPSPAPPPRRRR
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.84
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 5.55
PUBMED ID: N

Experiment series 5

Protein A

Protein: SRRM1
Fragment: 576-590
Site: PxxP_7
Sequence: PPRRRTPSPPPRRRS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.81
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.46
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: SRRM1
Fragment: 576-590
Site: PxxP_7
Sequence: PPRRRTPSPPPRRRS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.81
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 5.46
PUBMED ID: N

Experiment series 7

Protein A

Protein: SRRM1
Fragment: 544-558
Site: PxxP_4
Sequence: GRRRRSPSPPPTRRR
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.67
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 5.13
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: SRRM1
Fragment: 544-558
Site: PxxP_4
Sequence: GRRRRSPSPPPTRRR
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.67
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 5.13
PUBMED ID: N

Experiment series 9

Protein A

Protein: SRRM1
Fragment: 327-341
Site: PxxP_1
Sequence: TPPRRMPPPPRHRRS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.47
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.75
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: SRRM1
Fragment: 327-341
Site: PxxP_1
Sequence: TPPRRMPPPPRHRRS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.47
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.75
PUBMED ID: N

Experiment series 11

Protein A

Protein: SRRM1
Fragment: 621-635
Site: PxxP_11
Sequence: PKRRASPSPPPKRRV
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.38
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.56
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: SRRM1
Fragment: 621-635
Site: PxxP_11
Sequence: PKRRASPSPPPKRRV
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.38
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.56
PUBMED ID: N

Experiment series 13

Protein A

Protein: SRRM1
Fragment: 612-626
Site: PxxP_10
Sequence: RRTASPPPPPKRRAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.36
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.53
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: SRRM1
Fragment: 612-626
Site: PxxP_10
Sequence: RRTASPPPPPKRRAS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.36
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.53
PUBMED ID: N

Experiment series 15

Protein A

Protein: SRRM1
Fragment: 576-590
Site: PxxP_7
Sequence: PPRRRTPSPPPRRRS
Construct: Bio E E Ado Ado-PPRRRTPSPPPRRRS-CONH2

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.32
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: SRRM1
Fragment: 611-625
Site: PxxP_9
Sequence: KRRTASPPPPPKRRA
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.32
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.44
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: SRRM1
Fragment: 611-625
Site: PxxP_9
Sequence: KRRTASPPPPPKRRA
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.32
Holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.44
PUBMED ID: N

Experiment series 18

Protein A

Protein: SRRM1
Fragment: 395-409
Site: PxxP_3
Sequence: PRRRHRPSPPATPPP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.3
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.41
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: SRRM1
Fragment: 395-409
Site: PxxP_3
Sequence: PRRRHRPSPPATPPP
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.3
Holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
Measured pKd: 4.41
PUBMED ID: N

Experiment series 20

Protein A

Protein: SRRM1
Fragment: 600-614
Site: PxxP_8
Sequence: IQRRYSPSPPPKRRT
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.3
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.41
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: SRRM1
Fragment: 600-614
Site: PxxP_8
Sequence: IQRRYSPSPPPKRRT
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.3
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.41
PUBMED ID: N

Experiment series 22

Protein A

Protein: SRRM1
Fragment: 365-379
Site: PxxP_2
Sequence: SRSRSPPKKPPKRTS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.24
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.27
PUBMED ID: 38995680

Experiment series 23

Protein A

Protein: SRRM1
Fragment: 365-379
Site: PxxP_2
Sequence: SRSRSPPKKPPKRTS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.24
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 4
Measured pKd: 4.27
PUBMED ID: N

Experiment series 24

Protein A

Protein: SRRM1
Fragment: 1-904
Construct: endogenous protein (Jurkat)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.1
Immobilized partner concentration (10-6M): 10
P value: 4.46
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 25

Protein A

Protein: SRRM1
Fragment: 759-773
Site: PxxP_12
Sequence: PAAKKPPAPPSPVQS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 26

Protein A

Protein: SRRM1
Fragment: 759-773
Site: PxxP_12
Sequence: PAAKKPPAPPSPVQS
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.01
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N

Experiment series 27

Protein A

Protein: SRRM1
Fragment: 776-790
Site: PxxP_13
Sequence: PSTNWSPAVPVKKAK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 28

Protein A

Protein: SRRM1
Fragment: 776-790
Site: PxxP_13
Sequence: PSTNWSPAVPVKKAK
Construct: Biotinylated 15-mer peptide (crude)

Protein B

Protein: AMPH
Fragment: 615-695
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.03
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022-06-29
Number of measurements: 2
The affinity was below the detection threshold of the assay.
PUBMED ID: N