Interaction Details


Details of experiments between Q8IY67 and O00499
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RAVER1
Fragment: 1-606
Construct: endogenous protein (Jurkat)

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: avi(biotin)-his6-MBP-TEVsite-SH3
Average holdup BI: 0.63
Immobilized partner concentration (10-6M): 10
P value: 6.53
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-06-29
Number of measurements: 3
Measured pKd: 5.23
PUBMED ID: 38995680
Experimental Details

-log10(P Two-sided unpaired T-test (6 vs 3))

Experiment series 2

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.56
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.91
PUBMED ID: 38995680

Experiment series 3

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.02
PUBMED ID: 38995680

Experiment series 4

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.36
PUBMED ID: 38995680

Experiment series 5

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: D537V
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.77
PUBMED ID: 38995680

Experiment series 6

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 7

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: F584S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 8

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.33
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.84
PUBMED ID: 38995680

Experiment series 9

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: P551L
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 8
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.85
PUBMED ID: 38995680

Experiment series 10

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.6
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.97
PUBMED ID: 38995680

Experiment series 11

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Q540H
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.18
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.1
PUBMED ID: 38995680

Experiment series 12

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.37
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.83
PUBMED ID: 38995680

Experiment series 13

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: R581C
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 10
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680

Experiment series 14

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.57
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 4.92
PUBMED ID: 38995680

Experiment series 15

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: T532M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-16
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 38995680

Experiment series 16

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.59
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.96
PUBMED ID: 38995680

Experiment series 17

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V566M
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 38995680

Experiment series 18

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.4
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 4.77
PUBMED ID: 38995680

Experiment series 19

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: V583I
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.06
Immobilized partner concentration (10-6M): 13
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-17
Number of measurements: 1
Measured pKd: 3.67
PUBMED ID: 38995680

Experiment series 20

Protein A

Protein: RAVER1
Fragment: 420-434
Site: PxxP_1
Sequence: AGGGLPPELPPRRGK
Construct: Bio Ado Ado-AGGGLPPELPPRRGK-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: 0.24
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
Measured pKd: 4.26
PUBMED ID: 38995680

Experiment series 21

Protein A

Protein: RAVER1
Fragment: 431-445
Site: PxxP_2
Sequence: RRGKPPPLLPSVLGP
Construct: Bio Ado Ado-RRGKPPPLLPSVLGP-CONH2

Protein B

Protein: BIN1
Fragment: 513-593
Site: SH3
Modification: Y531S
Construct: his6-MBP-TEVsite-SH3
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: HU (DAPF)
Details of affinity fitting: Quadratic binding equation
Date of measurement: 2023-01-18
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 38995680