Interaction Details


Details of experiments between Q8IXQ8 and P03126
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A

Protein: PDZD9
Fragment: 23-120
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV16-E6
Fragment: 149-158
Site: PBM
Sequence: SSRTRRETQL
Construct: biotin-ttds-SSRTRRETQL
Average holdup BI: 0.14
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 15
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 5
Measured pKd: 4.06
PUBMED ID: 36115835