Interaction Details


Details of experiments between Q8IX15 and P31946
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HOMEZ
Fragment: 439-448
Site: 14-3-3_pSer444
Modification: (SEP)444
Sequence: PPSTRSLNER
Construct: PPSTR(pSer)LNER (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.38
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 102
P value: 4.6
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.78
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: HOMEZ
Fragment: 439-448
Site: 14-3-3_pSer444
Modification: (SEP)444
Sequence: PPSTRSLNER
Construct: PPSTR(pSer)LNER (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.38
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 134
P value: 3.71
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.66
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))