Interaction Details


Details of experiments between Q8IW70 and Q6ZMN7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: TMEM151B
Fragment: 557-566
Site: PBM
Sequence: RHGSCVETSL
Construct: biotin-ado-ado-RHGSCVETSL

Protein B

Protein: PDZRN4
Fragment: 214-318
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.66
Normalized holdup BI: 0.74
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.27
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.