Interaction Details


Details of experiments between Q8IVT5 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: KSR1
Fragment: 326-335
Site: 14-3-3_pSer331
Modification: (SEP)331
Sequence: MRFDLSHGSP
Construct: MRFDL(pSer)HGSP (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.05
Holdup BI standard deviation: 0.2
Immobilized partner concentration (10-6M): 130
P value: 0.22
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: KSR1
Fragment: 401-410
Site: 14-3-3_pSer406
Modification: (SEP)406
Sequence: LRRTESVPSD
Construct: LRRTE(pSer)VPSD (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.53
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 130
P value: 7.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.93
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: KSR1
Fragment: 268-277
Site: 14-3-3_pThr273
Modification: (TPO)273
Sequence: FITPPTTPQL
Construct: FITPP(pThr)TPQL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.07
Holdup BI standard deviation: 0.31
Immobilized partner concentration (10-6M): 130
P value: 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))