Interaction Details


Details of experiments between Q8IVF2 and Q9NYB5
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: AHNAK2
Fragment: 108-193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SLCO1C1
Fragment: 703-712
Site: PBM
Sequence: NYWPGKETQL
Construct: biotin-ttds-NYWPGKETQL
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.12
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 5.5
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.41
PUBMED ID: 36115835