Interaction Details


Details of experiments between Q86XP1 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: DGKH
Fragment: 1211-1220
Site: PBM
Sequence: LGRSTPQSEV
Construct: biotin-ado-ado-LGRSTPQSEV

Protein B

Protein: SNX27
Fragment: 40-141
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.89
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: DGKH
Fragment: 1211-1220
Site: PBM
Sequence: LGRSTPQSEV
Construct: biotin-ttds-LGRSTPQSEV

Protein B

Protein: SNX27
Fragment: 1-541
Construct: endogenous protein (Jurkat)
Average holdup BI: 0.09
Immobilized partner concentration (10-6M): 10
P value: 0.59
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Number of measurements: 3
The affinity was below the detection threshold of the assay.
PUBMED ID: 37942667
Experimental Details

-log10(P Two-sided unpaired T-test (3 vs 6))