Interaction Details


Details of experiments between Q86UT5 and P53778
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NHERF4
Fragment: 106-208
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0.26
Normalized holdup BI: 0.26
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.32
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: NHERF4
Fragment: 106-208
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.11
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.85
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: NHERF4
Fragment: 220-307
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: -0.01
Normalized holdup BI: -0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 4

Protein A

Protein: NHERF4
Fragment: 220-307
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: NHERF4
Fragment: 464-550
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: -0.05
Normalized holdup BI: -0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: NHERF4
Fragment: 464-550
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835