Interaction Details


Details of experiments between Q86UL8 and Q9IR59
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.86
Holdup BI standard deviation: 0.05
Normalized holdup BI: 0.95
Normalized holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.89
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.8
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.76
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.41
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.41
Normalized holdup BI: 0.57
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.21
Normalized holdup BI: 0.21
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.11
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.73
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.06
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.46
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MAGI2
Fragment: 1-102
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV82-E6
Fragment: 142-151
Site: PBM
Sequence: PPRQRSETQV
Construct: biotin-ttds-PPRQRSETQV
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835