Interaction Details


Details of experiments between Q86UL8 and Q8TDW7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV
Average holdup BI: 0.46
Holdup BI standard deviation: 0
Normalized holdup BI: 0.46
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.72
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV
Average holdup BI: 0.14
Normalized holdup BI: 0.15
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.02
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV
Average holdup BI: 0.09
Holdup BI standard deviation: 0
Normalized holdup BI: 0.12
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.91
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV
Average holdup BI: 0.08
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.09
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.77
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: FAT3
Fragment: 4548-4557
Site: PBM
Sequence: FVETQHQTQV
Construct: biotin-ttds-FVETQHQTQV
Average holdup BI: 0.06
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.07
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.63
PUBMED ID: 36115835