Interaction Details


Details of experiments between Q86UL8 and Q13574
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.28
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.89
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.21
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.72
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.37
Normalized holdup BI: 0.37
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.69
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.42
Normalized holdup BI: 0.42
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.64
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 5

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.28
Normalized holdup BI: 0.34
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.63
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.31
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.47
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 7

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.33
Normalized holdup BI: 0.33
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.46
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.22
Normalized holdup BI: 0.26
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.45
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.11
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.35
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Measured dissociation constant (10-6M): 66.29
Standard deviation of dissociation constant (10-6M): 7.34
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.18
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.13
Normalized holdup BI: 0.14
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.98
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 12

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.05
Holdup BI standard deviation: 0
Normalized holdup BI: 0.06
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.65
PUBMED ID: 36115835

Experiment series 13

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.04
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.5
PUBMED ID: 36115835
Experimental Details

not reported internal standards of holdup layout #6

Experiment series 14

Protein A

Protein: MAGI2
Fragment: 1-102
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 15

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 16

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 17

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: DGKZ
Fragment: 919-928
Site: PBM
Sequence: IQREDQETAV
Construct: biotin-ttds-IQREDQETAV
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835