Interaction Details


Details of experiments between Q86UL8 and P53778
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0.13
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.95
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0.12
Normalized holdup BI: 0.13
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.94
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.1
Normalized holdup BI: 0.12
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.89
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.1
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: -0.02
Normalized holdup BI: -0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 6

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.01
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: Biotin-ado-ado-GARVSKETPL
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 10

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: MAPK12
Fragment: 358-367
Site: PBM
Sequence: GARVSKETPL
Construct: biotin-ttds-GARVSKETPL
Average holdup BI: 0.01
Normalized holdup BI: 0.01
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835