Interaction Details


Details of experiments between Q86UL8 and P34998
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CRHR1
Fragment: 435-444
Site: PBM
Sequence: FHSIKQSTAV
Construct: biotin-ado-ado-FHSIKQSTAV
Average holdup BI: 0.56
Normalized holdup BI: 0.63
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.03
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 2

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CRHR1
Fragment: 435-444
Site: PBM
Sequence: FHSIKQSTAV
Construct: biotin-ado-ado-FHSIKQSTAV
Average holdup BI: 0.23
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.42
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 3

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CRHR1
Fragment: 435-444
Site: PBM
Sequence: FHSIKQSTAV
Construct: biotin-ado-ado-FHSIKQSTAV
Average holdup BI: 0.29
Normalized holdup BI: 0.29
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.38
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 4

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CRHR1
Fragment: 435-444
Site: PBM
Sequence: FHSIKQSTAV
Construct: biotin-ado-ado-FHSIKQSTAV
Average holdup BI: 0.11
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.83
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.

Experiment series 5

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CRHR1
Fragment: 435-444
Site: PBM
Sequence: FHSIKQSTAV
Construct: biotin-ado-ado-FHSIKQSTAV
Average holdup BI: 0.06
Normalized holdup BI: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.61
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.