Interaction Details


Details of experiments between Q86UL8 and P20020
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: Biotin-ado-ado-SPLHSLETSL
Average holdup BI: 0.31
Normalized holdup BI: 0.31
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.44
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 2

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: Biotin-ado-ado-SPLHSLETSL
Average holdup BI: 0.29
Normalized holdup BI: 0.29
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.39
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: biotin-ttds-SPLHSLETSL
Average holdup BI: 0.26
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.26
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.31
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: biotin-ttds-SPLHSLETSL
Average holdup BI: 0.19
Holdup BI standard deviation: 0
Normalized holdup BI: 0.23
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.24
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: biotin-ttds-SPLHSLETSL
Average holdup BI: 0.1
Normalized holdup BI: 0.11
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.86
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: Biotin-ado-ado-SPLHSLETSL
Average holdup BI: 0.07
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.7
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 7

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: Biotin-ado-ado-SPLHSLETSL
Average holdup BI: 0.06
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.56
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 8

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: Biotin-ado-ado-SPLHSLETSL
Average holdup BI: 0.04
Normalized holdup BI: 0.06
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.52
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 9

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: biotin-ttds-SPLHSLETSL
Average holdup BI: 0.04
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.5
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: ATP2B1
Fragment: 1211-1220
Site: PBM
Sequence: SPLHSLETSL
Construct: biotin-ttds-SPLHSLETSL
Average holdup BI: 0.04
Holdup BI standard deviation: 0.02
Normalized holdup BI: 0.05
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 3.48
PUBMED ID: 36115835