Interaction Details


Details of experiments between Q86UL8 and A4U7F2
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Measured dissociation constant (10-6M): 1.89
Standard deviation of dissociation constant (10-6M): 0.13
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 5.72
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.65
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.39
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: MAGI2
Fragment: 410-522
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.73
Normalized holdup BI: 0.8
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.32
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.16
Normalized holdup BI: 0.22
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.14
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.16
Normalized holdup BI: 0.16
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.94
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: MAGI2
Fragment: 1-102
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: -0.02
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: MAGI2
Fragment: 1141-1229
Site: PDZ_6
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 9

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.04
Normalized holdup BI: 0.04
Immobilized partner concentration (10-6M): 22
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 10

Protein A

Protein: MAGI2
Fragment: 600-682
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.04
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 11

Protein A

Protein: MAGI2
Fragment: 772-865
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.08
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 12

Protein A

Protein: MAGI2
Fragment: 915-1017
Site: PDZ_5
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: HPV67-E6
Fragment: 140-149
Site: PBM
Sequence: SRPQRTQTQV
Construct: biotin-ttds-SRPQRTQTQV
Average holdup BI: 0.07
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835