Interaction Details


Details of experiments between Q86SQ0 and P63104
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: PHLDB2
Fragment: 195-204
Site: 14-3-3_pSer200
Modification: (SEP)200
Sequence: RSGAASMPSS
Construct: RSGAA(pSer)MPSS (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.8
Holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 132
P value: 11.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.49
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: PHLDB2
Fragment: 207-216
Site: 14-3-3_pSer212
Modification: (SEP)212
Sequence: QARKMSIQDS
Construct: QARKM(pSer)IQDS (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.04
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 132
P value: 0.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: PHLDB2
Fragment: 272-281
Site: 14-3-3_pSer277
Modification: (SEP)277
Sequence: LPPRNSLGNS
Construct: LPPRN(pSer)LGNS (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.12
Holdup BI standard deviation: 0.04
Immobilized partner concentration (10-6M): 132
P value: 3.55
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: PHLDB2
Fragment: 410-419
Site: 14-3-3_pSer415
Modification: (SEP)415
Sequence: RERKSSISSI
Construct: RERKS(pSer)ISSI (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.15
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 132
P value: 1.37
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: PHLDB2
Fragment: 463-472
Site: 14-3-3_pSer468
Modification: (SEP)468
Sequence: PDSRLSTGTT
Construct: PDSRL(pSer)TGTT (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.06
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 132
P value: 0.88
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: PHLDB2
Fragment: 484-493
Site: 14-3-3_pSer489
Modification: (SEP)489
Sequence: EKLQLSDEES
Construct: EKLQL(pSer)DEES (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: -0.08
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 132
P value: 0.92
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: PHLDB2
Fragment: 1123-1132
Site: 14-3-3_pThr1128
Modification: (TPO)1128
Sequence: RSHVETAGHN
Construct: RSHVE(pThr)AGHN (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: -0.01
Holdup BI standard deviation: 0.46
Immobilized partner concentration (10-6M): 132
P value: 0.01
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 8

Protein A

Protein: PHLDB2
Fragment: 893-902
Site: 14-3-3_pThr898
Modification: (TPO)898
Sequence: LYLSDTLPRK
Construct: LYLSD(pThr)LPRK (crude synthetic peptide)

Protein B

Protein: YWHAZ
Fragment: 1-245
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3zeta
Average holdup BI: 0.09
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 132
P value: 1.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))