Interaction Details


Details of experiments between Q82005 and Q8TEW8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV

Protein B

Protein: PARD3B
Fragment: 376-471
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.14
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.24
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV

Protein B

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV

Protein B

Protein: PARD3B
Fragment: 184-299
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Normalized holdup BI: 0.02
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV73-E6
Fragment: 139-148
Site: PBM
Sequence: SGSRPSATVV
Construct: biotin-ttds-SGSRPSATVV

Protein B

Protein: PARD3B
Fragment: 490-594
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.04
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835