Interaction Details


Details of experiments between Q81964 and Q8N448
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Normalized holdup BI: 0.56
Immobilized partner concentration (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.04
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: LNX2
Fragment: 334-426
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.68
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: LNX2
Fragment: 592-688
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.61
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.56
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: LNX2
Fragment: 459-560
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835