Interaction Details


Details of experiments between Q81964 and Q6P0Q8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.55
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.55
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.03
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 12.7
Standard deviation of dissociation constant (10-6M): 0.74
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.9
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: MAST2
Fragment: 1102-1193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.85
PUBMED ID: 36115835