Interaction Details


Details of experiments between Q81964 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.58
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.75
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.45
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: DLG4
Fragment: 155-249
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.55
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.4
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.5
Holdup BI standard deviation: 0
Normalized holdup BI: 0.59
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 5.11
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.75
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 25.35
Standard deviation of dissociation constant (10-6M): 1.34
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
Number of measurements: 3
Measured pKd: 4.6
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: DLG4
Fragment: 62-152
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Immobilized partner concentration (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.59
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: HPV59-E6
Fragment: 151-160
Site: PBM
Sequence: QRQARSETLV
Construct: biotin-ttds-QRQARSETLV

Protein B

Protein: DLG4
Fragment: 306-403
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.15
Holdup BI standard deviation: 0.06
Normalized holdup BI: 0.17
Normalized holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 13.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 4
Measured pKd: 4.21
PUBMED ID: 36115835