Interaction Details


Details of experiments between Q7Z6J2 and Q9Y6N9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRASP
Fragment: 96-193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: USH1C
Fragment: 543-552
Site: PBM
Sequence: KEYDDELTFF
Construct: Biotin-Ahx-KEYDDELTFF
Average holdup BI: 0.3
Normalized holdup BI: 0.3
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.41
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: USH1C
Fragment: 208-299
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: USH1C
Fragment: 441-548
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: USH1C
Fragment: 75-178
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835