Interaction Details


Details of experiments between Q7Z6J2 and Q9UPX8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: SHANK2
Fragment: 239-346
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.17
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.33
PUBMED ID: 36115835