Interaction Details


Details of experiments between Q7Z6J2 and Q9UBN1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRASP
Fragment: 96-193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: CACNG4
Fragment: 318-327
Site: PBM
Sequence: SMLNRRTTPV
Construct: biotin-ado-ado-SMLNRRTTPV
Average holdup BI: 0.25
Holdup BI standard deviation: 0
Normalized holdup BI: 0.25
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.3
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.