Interaction Details


Details of experiments between Q7Z6J2 and Q6P9H4
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: CNKSR3
Fragment: 203-303
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M):
Standard deviation of dissociation constant (10-6M): 0
Average holdup BI: 0.26
Normalized holdup BI: 0
Normalized holdup BI standard deviation: 0
Immobilized partner concentration (10-6M): 10
P value: 0
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.6
PUBMED ID: 36115835