Interaction Details


Details of experiments between Q7Z6J2 and Q5T2W1
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.69
Normalized holdup BI: 0.74
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.27
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: NHERF3
Fragment: 2-107
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.51
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.12
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: NHERF3
Fragment: 132-224
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.02
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: NHERF3
Fragment: 238-329
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.03
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 7

Protein A

Protein: GRASP
Fragment: 386-395
Site: PBM
Sequence: RSLEEEESQL
Construct: biotin-ttds-RSLEEEESQL

Protein B

Protein: NHERF3
Fragment: 375-459
Site: PDZ_4
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 8

Protein A

Protein: GRASP
Fragment: 96-193
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: NHERF3
Fragment: 510-519
Site: PBM
Sequence: SSSNSEDTEM
Construct: Biotin-Ahx-SSSNSEDTEM
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835