Interaction Details


Details of experiments between Q7Z628 and O15085
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV

Protein B

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.22
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 2
Measured pKd: 4.23
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: Biotin-ado-ado-SGGKRKETLV

Protein B

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.08
Immobilized partner concentration (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 3.68
PUBMED ID: 36115835
Experimental Details

Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6

Experiment series 3

Protein A

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV

Protein B

Protein: ARHGEF11
Fragment: 41-126
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0
Normalized holdup BI: -0
Immobilized partner concentration (10-6M): 17.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experimental Details

not reported internal standards of holdup layout #5