Interaction Details


Details of experiments between Q7Z628 and O14745
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV

Protein B

Protein: NHERF1
Fragment: 10-98
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.03
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: NET1
Fragment: 587-596
Site: PBM
Sequence: SGGKRKETLV
Construct: biotin-ttds-SGGKRKETLV

Protein B

Protein: NHERF1
Fragment: 146-237
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.09
Immobilized partner concentration (10-6M): 35
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
Number of measurements: 1
The affinity was below the detection threshold of the assay.
PUBMED ID: 36115835