Interaction Details


Details of experiments between Q7Z3C6 and P31946
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: ATG9A
Fragment: 9-18
Site: 14-3-3_pSer14
Modification: (SEP)14
Sequence: QRLEASYSDS
Construct: QRLEA(pSer)YSDS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.06
Holdup BI standard deviation: 0.05
Immobilized partner concentration (10-6M): 134
P value: 1.31
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 2

Protein A

Protein: ATG9A
Fragment: 9-18
Site: 14-3-3_pSer14
Modification: (SEP)14
Sequence: QRLEASYSDS
Construct: QRLEA(pSer)YSDS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: -0.08
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 102
P value: 1.25
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: ATG9A
Fragment: 736-745
Site: 14-3-3_pSer741
Modification: (SEP)741
Sequence: DESGESAPDE
Construct: DESGE(pSer)APDE (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.5
Holdup BI standard deviation: 0.31
Immobilized partner concentration (10-6M): 102
P value: 1.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.99
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: ATG9A
Fragment: 736-745
Site: 14-3-3_pSer741
Modification: (SEP)741
Sequence: DESGESAPDE
Construct: DESGE(pSer)APDE (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.39
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 134
P value: 3.28
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 3.68
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 5

Protein A

Protein: ATG9A
Fragment: 750-759
Site: 14-3-3_pSer755
Modification: (SEP)755
Sequence: ARAPQSIPRS
Construct: ARAPQ(pSer)IPRS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.18
Holdup BI standard deviation: 0.03
Immobilized partner concentration (10-6M): 134
P value: 4
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 6

Protein A

Protein: ATG9A
Fragment: 750-759
Site: 14-3-3_pSer755
Modification: (SEP)755
Sequence: ARAPQSIPRS
Construct: ARAPQ(pSer)IPRS (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.18
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 102
P value: 2.79
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: ATG9A
Fragment: 756-765
Site: 14-3-3_pSer761
Modification: (SEP)761
Sequence: IPRSASYPCA
Construct: IPRSA(pSer)YPCA (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.92
Holdup BI standard deviation: 0.06
Immobilized partner concentration (10-6M): 134
P value: 2.2
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022-02-17
Number of measurements: 4
Measured pKd: 4.95
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 4))

Experiment series 8

Protein A

Protein: ATG9A
Fragment: 756-765
Site: 14-3-3_pSer761
Modification: (SEP)761
Sequence: IPRSASYPCA
Construct: IPRSA(pSer)YPCA (crude synthetic peptide)

Protein B

Protein: YWHAB
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3beta
Average holdup BI: 0.18
Holdup BI standard deviation: 0.33
Immobilized partner concentration (10-6M): 102
P value: 0.41
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))