Interaction Details


Details of experiments between Q7KZI7 and Q04917
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: MARK2
Fragment: 404-413
Site: 14-3-3_pSer409
Modification: (SEP)409
Sequence: KQRRFSDQAA
Construct: KQRRF(pSer)DQAA (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.57
Holdup BI standard deviation: 0.11
Immobilized partner concentration (10-6M): 130
P value: 4.74
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 2

Protein A

Protein: MARK2
Fragment: 488-497
Site: 14-3-3_pSer493
Modification: (SEP)493
Sequence: LLERASLGQA
Construct: LLERA(pSer)LGQA (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.09
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 1.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 3

Protein A

Protein: MARK2
Fragment: 566-575
Site: 14-3-3_pSer571
Modification: (SEP)571
Sequence: PVASPSAHNI
Construct: PVASP(pSer)AHNI (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: -0.01
Holdup BI standard deviation: 0.15
Immobilized partner concentration (10-6M): 130
P value: 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 4

Protein A

Protein: MARK2
Fragment: 626-635
Site: 14-3-3_pSer631
Modification: (SEP)631
Sequence: GRRGASGSIF
Construct: GRRGA(pSer)GSIF (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.36
Holdup BI standard deviation: 0.07
Immobilized partner concentration (10-6M): 130
P value: 4.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 3.64
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 5

Protein A

Protein: MARK2
Fragment: 319-328
Site: 14-3-3_pThr324
Modification: (TPO)324
Sequence: NEVMATYLLL
Construct: NEVMA(pThr)YLLL (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.03
Holdup BI standard deviation: 0.53
Immobilized partner concentration (10-6M): 130
P value: 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 6

Protein A

Protein: MARK2
Fragment: 461-470
Site: 14-3-3_pThr466
Modification: (TPO)466
Sequence: LERKKTTPTP
Construct: LERKK(pThr)TPTP (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.05
Holdup BI standard deviation: 0.09
Immobilized partner concentration (10-6M): 130
P value: 0.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
The affinity was below the detection threshold of the assay.
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))

Experiment series 7

Protein A

Protein: MARK2
Fragment: 53-62
Site: 14-3-3_pThr58
Modification: (TPO)58
Sequence: YRLLKTIGKG
Construct: YRLLK(pThr)IGKG (crude synthetic peptide)

Protein B

Protein: YWHAH
Fragment: 1-246
Site: 14-3-3
Construct: avi-his6-MBP-TEVsite-14-3-3eta
Average holdup BI: 0.61
Holdup BI standard deviation: 0.08
Immobilized partner concentration (10-6M): 130
P value: 5.75
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021-10-13
Number of measurements: 5
Measured pKd: 4.08
DOI: 10.1101/2022.12.08.519103
Experimental Details

-log10(P Two-sided unpaired T-test (4 vs 5))