Interaction Details

Details of experiments between Q76G19 and P0DTC3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.

Experiment series 1

Protein A

Protein: PDZD4
Fragment: 117-226
Site: PDZ
Construct: his6-MBP-TEVsite-PDZ

Protein B

Protein: SARS-Cov2-3a
Fragment: 266-275
Site: PBM
Sequence: EPTTTTSVPL
Construct: Biotin-(PEG)3-YDEPTTTTSVPL

Average holdup BI: -0.04

Holdup BI standard deviation: 0.01

Immobilized partner concentration (10^-6M): 20

Experiment method: CALIP_holdup

Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout

Number of measurements: 2

The affinity was below the detection threshold of the assay.

PUBMED ID: 36115835