Interaction Details


Details of experiments between Q6ZTQ3 and Q12959
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.77
Normalized holdup BI: 0.86
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 5.55
PUBMED ID: 36115835

Experiment series 2

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.48
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.12
PUBMED ID: 36115835

Experiment series 3

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG1
Fragment: 314-412
Site: PDZ_2
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.48
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 5.11
PUBMED ID: 36115835

Experiment series 4

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG1
Fragment: 221-314
Site: PDZ_1
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.49
Normalized holdup BI: 0.58
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.88
PUBMED ID: 36115835

Experiment series 5

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.16
Immobilized partner concentration (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
Number of measurements: 1
Measured pKd: 4.37
PUBMED ID: 36115835

Experiment series 6

Protein A

Protein: RASSF6
Fragment: 360-369
Site: PBM
Sequence: KLVIKTETTV
Construct: biotin-ttds-KLVIKTETTV

Protein B

Protein: DLG1
Fragment: 457-570
Site: PDZ_3
Construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Normalized holdup BI: 0.24
Immobilized partner concentration (10-6M): 20.7
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Number of measurements: 1
Measured pKd: 4.2
PUBMED ID: 36115835